|
| Status |
Public on Feb 24, 2021 |
| Title |
C1#1 TH D50 n1 |
| Sample type |
RNA |
| |
|
| Source name |
Control
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC-derived dopaminergic neurons
genotype: control
|
| Growth protocol |
Human iPSC were routinely cultured in mTESR medium on matrigel coated plates. Directed differentiation of iPSC onto ventral dopaminergic neurons was carried out following a previously published protocol (Kriks, et al. Nature 480, 547-551; 2011), with minor modifications. Neurons were cultured for 50 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Quiazol lysis reagent (Qiagen) and RNAeasy extraction kit (Qiagen) according to manufacturer's instructions.
|
| Label |
n/a
|
| Label protocol |
nCounter Human Neuropathology Panel: XT-CSO-HNROP1-12
|
| |
|
| Hybridization protocol |
All the steps were carried out in the ice and using the nCounter nanoString reagent for the Neuropathology panel. The master mix for the hybridization process was prepared by mixing hybridization buffer and reporter CodeSet. A dilution of 10μg/μl for each RNA to be analyzed was prepared and maintained in ice. For each sample, a tube was prepared with 5μl of RNA (10μg/μl) and 8μl of master mix. 2μl of Capture ProbeSet was added in each tube and the hybridization process was started using a PCR machine at 65ºC for at least 16 hours.
|
| Scan protocol |
nSolver™ Analysis Software, Advanced Analysis Modules
|
| Description |
Skin Fibroblasts
Nanostring nCounter® Human Neuropathology Panel
|
| Data processing |
nSolver™ Analysis Software, Advanced Analysis Modules
|
| |
|
| Submission date |
Feb 23, 2021 |
| Last update date |
Feb 24, 2021 |
| Contact name |
Meritxell Pons |
| E-mail(s) |
meritxell.pons@ub.edu
|
| Organization name |
IDIBELL
|
| Lab |
Stem Cells and Neurodegeneration Lab
|
| Street address |
Avinguda Gran Via, 199-203
|
| City |
Hospitalet de Llobregat (Barcelona) |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL29761 |
| Series (1) |
| GSE167335 |
Gene expression profile of iPSC-derived dopaminergic neurons from control and Parkinson's disease patients carrying LRRK2 G2019S point mutation |
|
