|
| Status |
Public on Aug 03, 2021 |
| Title |
A-673/TR/shR2_1#10_Dox (-), 1 biological Rep, 72h |
| Sample type |
RNA |
| |
|
| Source name |
shRNA against RRM2 CDS region 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A-673
treatment: no dox, 72h
cell type: Ewing Sarcoma
|
| Treatment protocol |
Cells were treated with/without doxycycline (0.1 μg/ml) for 72h or 96h with a refreshment after 48h.
|
| Growth protocol |
100.000-200.000 cells were cultured in T-25 flasks over 72h for A-673 and 96h for ES7.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNA isolation kits from Macherey-Nagel.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Affymetrix GeneChip® WT PLUS Reagent Kit according to its manufacture's instruction from 6 μg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Clariom D™ Arrays. Microarrays were washed and stained with Affymetrix GeneChip® 3000 Fluidics Station.
|
| Scan protocol |
Microarrays were scanned with Affymetrix GeneChip® 3000 Scanner.
|
| Description |
A-673/TR/shR2_1 Dox (-)
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 09, 2021 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Thomas Georg Philipp Grunewald |
| E-mail(s) |
t.gruenewald@kitz-heidelberg.de
|
| Phone |
0049-6221-423718
|
| Organization name |
German Cancer Research Center (DKFZ)
|
| Department |
Division of Translational Pediatric Sarcoma Research
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE166415 |
Gene expression of two Ewing sarcoma cell lines (A-673, ES7), transduced with inducible shControl or two different shRNA against RRM2 |
|
