|
| Status |
Public on Nov 07, 2022 |
| Title |
WT whole rep2 |
| Sample type |
SRA |
| |
|
| Source name |
WT whole epididymis
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: wild type
age: adult
tissue: whole epididymis
|
| Treatment protocol |
All animals were housed in a standard specific pathogen-free animal house, kept on a 12-hour light/dark cycle, and received sterilised food and autoclaved water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
prefrontal cortex were rharvested , flash frozen on dry ice, and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
WT-2
|
| Data processing |
We applied the reads filtering towards the raw reads after sequencing to achieve the clean data following the criteria: a)10% base quality <15 b) 13% base quality <20.
Hisat2 2.0.4 mapping to reference genome
count counting
Genome_build: GRCm38
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Feb 04, 2021 |
| Last update date |
Nov 07, 2022 |
| Contact name |
Winnie Shum |
| Organization name |
ShanghaiTech University
|
| Street address |
393 Hua Xia Middle Rd. Pudong District
|
| City |
Shanghai |
| ZIP/Postal code |
201210 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE166141 |
Epididymal epithelial degeneration and lipid metabolism impairment account for male infertility in occludin knockout mice |
|
| Relations |
| BioSample |
SAMN17776825 |
| SRA |
SRX10020896 |
