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Sample GSM5031851 Query DataSets for GSM5031851
Status Public on Nov 10, 2021
Title Sample_ED_18h_Sham
Sample type SRA
 
Source name 18h - sham - S5'
Organism Xenopus laevis
Characteristics tissue: Caudal section of spinal cord
Stage: NF stage 50
surgery: sham
time of isolation after surgery (hours): 18
treatment: --
Extracted molecule total RNA
Extraction protocol Spinal cords from animals at R-stage were dissected in time-series after surgery and immediately stored in RNAlater. Posterioly the purification of total RNA was performed using the RNeasy Mini Kit.
RNA libraries were prepared by TruSeq RNA Library Prep Kit v2. Posterioly these were sequenced on the Illumina HiSeq 2000 and HiSeq 4000.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Illumina Casava 1.7 software was used for basecalling.
Paired-end reads were mapped to Xenopus laevis model transcriptome v9.1 (Xenbase) using Bowtie. Gene expression level was quantified by RSEM.
 
Submission date Jan 22, 2021
Last update date Nov 10, 2021
Contact name Juan LarraĆ­n
E-mail(s) jlarrain@bio.puc.cl
Organization name Pontificia Universidad Catolica de Chile
Department Cellular and Molecular Biology
Lab Developmental and Regenerative Biology
Street address Portugal 49
City Santiago
ZIP/Postal code 8330025
Country Chile
 
Platform ID GPL17682
Series (1)
GSE165343 High expression profiling analysis of the early response to spinal cord injury identified a key role for mTORC1 signaling
Relations
BioSample SAMN17497027
SRA SRX9921026

Supplementary file Size Download File type/resource
GSM5031851_Sample_ED_18h_Sham.genes.results.txt.gz 401.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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