|
| Status |
Public on Jan 05, 2021 |
| Title |
NU01-EiPS07_p13 |
| Sample type |
genomic |
| |
|
| Source name |
Urine
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human induced pluripotent stem cells
cell line: NU01hES01-EiPS07
time: p13
|
| Treatment protocol |
None
|
| Growth protocol |
Cell lines were grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from mononuclear cell preparations of leukemic blasts obtained from peripheral blood or bone marrow at diagnosis using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
The array image was acquired using Affymetrix Chromosome Anaysis Suite Software (ChAS version 4.0).
Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
| |
|
| Submission date |
Dec 14, 2020 |
| Last update date |
Jan 05, 2021 |
| Contact name |
Kim Jung-Hyun |
| E-mail(s) |
dr.junghkim@gmail.com
|
| Organization name |
Korea National Institute of Health
|
| Department |
Division of Intractable Diseases
|
| Street address |
202, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu
|
| City |
Cheongju-si |
| ZIP/Postal code |
28160 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16131 |
| Series (1) |
| GSE163134 |
SNParray data for banking lines in Korea National Stem Cell Bank |
|
