tissue: Colorecal Cancer treatment response: FL_Responder
Extracted molecule
total RNA
Extraction protocol
Surgically isolated colorectal cancers were immediately frozen in liquid nitrogen. Tissues were lysed and total RNA was isolated using RNeasy Mini Kit (QIAGEN, Chatswort,CA) according to the manufacturer's instructions.
Label
biotin
Label protocol
Approximately 8 ug of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
FL_Responder
Data processing
Affymetrix Microarray Suite version 5.0, PerChip/PerGene normalization