|
| Status |
Public on Feb 01, 2021 |
| Title |
WT_Mock_4 |
| Sample type |
SRA |
| |
|
| Source name |
whole adult midgut
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: midgut
genotype: UAS-Dcr2 / w1118; esg-Gal4NP5130, UAS-GFP, UAS-GFPnLacZ / +; tub-Gal80ts / +
treatment: Mock treated (5% sucrose)
age: 10-14 days after eclosion
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Extracted midguts were frozen on dry ice then RNA was extracted using the QIAGEN RNAeasy kit with on column DNase digestion.
RNA libraries were prepared for sequencing using Illumina TrueSeq RNA Library Preparation Kit v2 following standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
MNR22170301_S8_R1_001_MN08_WT_S08_R1_001_MN08_WT_SUC4
|
| Data processing |
Quality checks were done using fastqc version 0.11.7 and fastqc_screen version 0.12.0
Reads were aligned using HiSat2 version 2.1.0
Differential gene expression analysis was based on the negative binomial distribution using DESeq2 package version 1.18.1
Genome_build: dm6 / BDGP 6
Supplementary_files_format_and_content: xlsx, expression levels and differential gene expression between different conditions
|
| |
|
| Submission date |
Dec 01, 2020 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Mate Naszai |
| Organization name |
University of Glasgow
|
| Street address |
Wolfson Wohl Cancer Research Centre
|
| City |
Glasgow |
| ZIP/Postal code |
G61 1QH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE162421 |
RAL GTPases mediate EGFR/MAPK signalling-driven intestinal stem cell proliferation and tumourigenesis upstream of RAS activation. |
|
| Relations |
| BioSample |
SAMN16969311 |
| SRA |
SRX9613379 |
