|
| Status |
Public on Dec 30, 2009 |
| Title |
IP-1.1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Ink4a/Arf-/- Pten-/- mouse astrocytes
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Ink4a/Arf-/- Pten-/- mouse astrocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the HiPure miRNA Isolation Kit (Roche)
|
| Label |
Hy3
|
| Label protocol |
5'-phosphtes were removed from terminal of microRNAs by Calf Intestinal Alkaline Phosphatase then Hy3/Hy5 fluorescent labels were attached enzymatically to the 3’-end of the miRNAs in the total RNA sample.
|
| |
|
| Channel 2 |
| Source name |
Common reference Pool
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Common reference Pool
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the HiPure miRNA Isolation Kit (Roche)
|
| Label |
Hy5
|
| Label protocol |
5'-phosphtes were removed from terminal of microRNAs by Calf Intestinal Alkaline Phosphatase then Hy3/Hy5 fluorescent labels were attached enzymatically to the 3’-end of the miRNAs in the total RNA sample.
|
| |
|
| |
| Hybridization protocol |
Labeled miRNA were Hybridize at 56°C for 16 hours on Exiqon V. 11.0 miRCURY LNA microRNA platform. Platforms were washed and stained using Tecan HS480 Pro automated hybridization stations.
|
| Scan protocol |
Platforms were scanned using the Agilent G2505B Microarray Scanners.
|
| Description |
microRNA expression
|
| Data processing |
Data were normalized using non-linear regression method (LOESS), and unsupervised hierarchical clustering was performed on log2(Hy3/Hy5); ΔLogMediaRatio > 0.50.
|
| |
|
| Submission date |
Dec 28, 2009 |
| Last update date |
Dec 31, 2009 |
| Contact name |
Hailei Zhang |
| E-mail(s) |
hailei_zhang@dfci.harvard.edu
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
44 Binney Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (2) |
| GSE19692 |
microRNA expression of Ink4a/Arf-/- Pten-/- mouse astrocytes |
| GSE19693 |
STAR RNA-binding protein, Quaking, suppresses cancer via regulation of microRNA |
|
