|
| Status |
Public on Jan 31, 2021 |
| Title |
Metapolybia_Worker |
| Sample type |
SRA |
| |
|
| Source name |
Whole brain
|
| Organism |
Metapolybia cingulata |
| Characteristics |
caste: Worker
tissue: whole brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All wasps were collected directly from their nests during the daytime, placed immediately into RNAlater (Ambion, Invitrogen) and stored at -20°C until further use. Total RNA was extracted using the RNeasy Universal Plus Mini kit (Qiagen, #73404)
RNA libraries were prepared for sequencing using standard Illumina Truseq kit protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Trinity used to assemble a de novo transcriptome
RSEM used to calculate normalised gene counts per Trinity transcript
Supplementary_files_format_and_content: Trinity fasta transcriptome files (.fnn) and tab-delimited RSEM files for each queen/worker which include TPM/FPKM and expected count for each sample.
|
| |
|
| Submission date |
Oct 23, 2020 |
| Last update date |
Jan 31, 2021 |
| Contact name |
Christopher Douglas Robert Wyatt |
| E-mail(s) |
cw13722@gmail.com
|
| Organization name |
UCL
|
| Street address |
Gower street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL29285 |
| Series (1) |
| GSE159973 |
Genetic toolkit for sociality predicts castes across the spectrum of social complexity in wasps |
|
| Relations |
| BioSample |
SAMN16532965 |
| SRA |
SRX9350988 |
