Data table header descriptions |
ID_REF |
|
X |
the X-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image. |
Y |
the Y-coordinate in µm of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image. |
Dia. |
the diameter in µm of the feature-indicator. |
F635 Median |
median feature pixel intensity at wavelength 1 (635 nm). |
F635 Mean |
mean feature pixel intensity at wavelength 1 (635 nm). |
F635 SD |
the standard deviation of the feature pixel intensity at wavelength 1 (635 nm). |
B635 Median |
the median feature background intensity at wavelength 1 (635 nm). |
B635 Mean |
the mean feature background intensity at wavelength 1 (635 nm). |
B635 SD |
|
% > B635+1SD |
|
% > B635+2SD |
|
F635 % Sat. |
|
F532 Median |
median feature pixel intensity at wavelength 2 (532 nm). |
F532 Mean |
mean feature pixel intensity at wavelength 2 (532 nm). |
F532 SD |
|
B532 Median |
the median feature background intensity at wavelength 2 (532 nm). |
B532 Mean |
the mean feature background intensity at wavelength 2 (532 nm). |
B532 SD |
the standard deviation of the feature background intensity at wavelength 2 (532 nm). |
% > B532+1SD |
|
% > B532+2SD |
|
F532 % Sat. |
the percentage of feature pixels at wavelength 2 that are saturated. |
Ratio of Medians (635/532) |
the ratio of the median intensities of each feature for each wavelength, with the median background subtracted. |
Ratio of Means (635/532) |
the ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted. |
Median of Ratios (635/532) |
the median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted. |
Mean of Ratios (635/532) |
the geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted. |
Ratios SD (635/532) |
the geometric standard deviation of the pixel intensity ratios. |
Rgn Ratio (635/532) |
the regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature. |
Rgn R² (635/532) |
the coefficient of determination for the current regression value. |
F Pixels |
the total number of feature pixels. |
B Pixels |
the total number of background pixels. |
Sum of Medians |
the sum of the median intensities for each wavelength, with the median background subtracted. |
Sum of Means |
the sum of the arithmetic mean intensities for each wavelength, with the median background subtracted. |
VALUE |
log (base 2) transform of the ratio of the medians. |
F635 Median - B635 |
the median feature pixel intensity at wavelength 1 with the median background subtracted. |
F532 Median - B532 |
the median feature pixel intensity at wavelength 2 with the median background subtracted. |
F635 Mean - B635 |
the mean feature pixel intensity at wavelength 1 with the median background subtracted. |
F532 Mean - B532 |
the mean feature pixel intensity at wavelength 2 with the median background subtracted. |
F635 Total Intensity |
Total Intensity = the sum of feature pixel intensities at wavelength 1 |
F532 Total Intensity |
Total Intensity = the sum of feature pixel intensities at wavelength 1 |
SNR 635 |
the signal-to-noise ratio at wavelength 1, defined by (Mean Foreground 1- Mean Background 1) / (Standard deviation of Background 1) |
SNR 532 |
the signal-to-noise ratio at wavelength 1, defined by (Mean Foreground 1- Mean Background 1) / (Standard deviation of Background 1) |
Flags |
the type of flag associated with a feature. |
Normalize |
the normalization status of the feature (included/not included). |