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Sample GSM478097 Query DataSets for GSM478097
Status Public on Mar 01, 2010
Title 1_6_IL17_1h_rep1
Sample type RNA
Source name primary murine hepatocytes cultivated on collagen coated plates
Organism Mus musculus
Characteristics tissue: liver
gender: male
agent: IL17
time: 1h
Extracted molecule total RNA
Extraction protocol RNA was prepared from cultured hepatocytes with the Qiagen RNEasy plus Kit according to the manufactures instructions. For each sample, 2 million cells were used for RNA isolation and purification. Samples for microarray analysis were subjected to a quality control using an Agilent Bioanalyzer with the Pico RNA-Kit. Only RNA with a RIN better than 8.2 was used for labeling and subsequent hybridization.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3001
Description Gene expression data from primary mouse hepatocytes
Data processing Mouse430V2 Affymetrix CEL data files were uploaded into Expressionist Refiner Array® version 4.5 (Genedata AG, Basel, Switzerland) for data condensation. Oligo hybridization intensities were condensed to probe set expression values using the MAS5 Affymetrix Statistical algorithm according to [60]. Condensed data were normalized using Expressionist Analyst® version 4.5 (Genedata AG, Basel, Switzerland). The mapping from probe-sets to genes was performed using the Phylosopher seqmap tool based on the NCBI mouse genome version 33. Absolute probe set expression values were scaled on the chip level by logarithmic mean normalization (for each chip, the geometric mean of all probe set values was scaled to 10000).
Submission date Dec 02, 2009
Last update date Jan 22, 2010
Contact name Irmgard Merfort
Organization name University of Freiburg - Institute of Pharmaceutical Science
Department Department of Pharmaceutical Biology and Biotechnology
Lab Merfort
Street address Stefan-Meier-Str. 19
City Freiburg
State/province BW
ZIP/Postal code 79104
Country Germany
Platform ID GPL1261
Series (1)
GSE19272 Genome-wide comparison between IL-17 and combined TNF-alpha/ IL-17 induced genes in primary murine hepatocytes

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 75389
AFFX-BioB-M_at 106876
AFFX-BioC-5_at 208196
AFFX-BioDn-5_at 424934
AFFX-CreX-5_at 2010891
AFFX-CreX-3_at 2534104
AFFX-ThrX-5_at 56626
AFFX-b-ActinMur/M12481_5_at 3685778
AFFX-b-ActinMur/M12481_M_at 3636621
AFFX-b-ActinMur/M12481_3_at 4435932
AFFX-GapdhMur/M32599_5_at 4327343
AFFX-GapdhMur/M32599_M_at 3872643
AFFX-GapdhMur/M32599_3_at 3282535
AFFX-TransRecMur/X57349_5_at 76809
AFFX-TransRecMur/X57349_M_at 50593
AFFX-TransRecMur/X57349_3_at 139309
AFFX-PyruCarbMur/L09192_5_at 47773
AFFX-PyruCarbMur/L09192_MA_at 65499
AFFX-PyruCarbMur/L09192_MB_at 70442
AFFX-PyruCarbMur/L09192_3_at 133193

Total number of rows: 45101

Table truncated, full table size 743 Kbytes.

Supplementary file Size Download File type/resource
GSM478097.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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