|
| Status |
Public on Jan 12, 2021 |
| Title |
A. aculeatus BCC199 condition A2 [BCC199_lot1_A2] |
| Sample type |
SRA |
| |
|
| Source name |
Technical replicate of lot1 BCC199
|
| Organism |
Aspergillus aculeatus |
| Characteristics |
strain: BCC199
age: 72 hours, 30 degree Celsius
medium: Wheat bran and soy bean
|
| Growth protocol |
Wheat bran and Soy bean mill
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Fungal cell were cultured for 3 days at 30 degree, frozen with liquid nitrogen, and RNA was harvested using Trizol reagent, following RNAeasy kit (Qiagen), contaminated DNA was eliminate by RNAase free DNase kit (Qiagen). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Sequencing, and bioinformatic analyses were performed by Novogene, Inc.
Illumina CASAVA 1.8 software was used for basecalling.
Sequenced reads for adaptor sequence, and low-quality sequence were trimmed and removed using Fastp.
Genome_build: NOT PROVIDED; REQUESTED
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Sep 09, 2020 |
| Last update date |
Jan 12, 2021 |
| Contact name |
Wuttichai Mhuantong |
| E-mail(s) |
larukuka@gmail.com
|
| Organization name |
BIOTEC
|
| Street address |
National Center for Genetic Engineering and Biotechnology (BIOTEC)
|
| City |
Khlong Luang |
| State/province |
Pathum Thani |
| ZIP/Postal code |
12120 |
| Country |
Thailand |
| |
|
| Platform ID |
GPL29120 |
| Series (1) |
| GSE157700 |
Transcriptome analysis and comparison of three Aspergillus aculeatus strains |
|
| Relations |
| BioSample |
SAMN16086472 |
| SRA |
SRX9098790 |
