|
| Status |
Public on May 07, 2010 |
| Title |
NSCLC EMC TB0032tu |
| Sample type |
RNA |
| |
|
| Source name |
frozen tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: tumor
cell type: LCC
overall survival: 50.53
status: alive
gender: M
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station Fluidics 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray scanner 3000
|
| Description |
Gene expression data from primary NSCLC
|
| Data processing |
The data was normalized by Robust Multi-Array average (RMA) algorithm. Intensities of probe sets lower than 30 were reset to 30.The intensity values of individual probe sets in each sample were then displayed as the log 2 of the deviations to the calculated geometric means for that probe sets.
|
| |
|
| Submission date |
Nov 25, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Sjaak Philipsen |
| E-mail(s) |
j.philipsen@erasmusmc.nl
|
| Phone |
+31-10-7044282
|
| Fax |
+31-10-7044743
|
| URL |
http://www.erasmusmc.nl/medical_genetics/
|
| Organization name |
Erasmus MC
|
| Department |
Cell Biology
|
| Street address |
Dr. Molewaterplein 50
|
| City |
Rotterdam |
| ZIP/Postal code |
3015GE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19188 |
Expression data for early stage NSCLC |
|
| Relations |
| Reanalyzed by |
GSE60486 |
| Reanalyzed by |
GSE119087 |
