|
| Status |
Public on Aug 08, 2020 |
| Title |
HCT116_H3.3WT |
| Sample type |
genomic |
| |
|
| Source name |
HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
compound: pCDH_H3.3WT_FLAG_HA
|
| Treatment protocol |
none
|
| Growth protocol |
37ºC, 5% CO2, McCoy's 5A (HCT116) or DMEM (HeLa) media with 10% FBS and no antibiotics
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen Dneasy kit
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to the Illumina Infinium MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using iScan using standard recommended Illumina scanner setting
|
| Data processing |
openSesame from SeSAMe R package
|
| |
|
| Submission date |
Aug 07, 2020 |
| Last update date |
Aug 08, 2020 |
| Contact name |
Scott Rothbart |
| E-mail(s) |
scott.rothbart@vai.org
|
| Organization name |
Van Andel Research Institute
|
| Department |
Center for Epigenetics
|
| Lab |
Rothbart Lab
|
| Street address |
333 Bostwick Ave NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL23976 |
| Series (1) |
| GSE148086 |
DNA methylation analysis of HCT116 after disruption of methyllysine reading and writing. |
|
