|
| Status |
Public on Nov 13, 2009 |
| Title |
WT-H2O2 treated-1 versus WT-untreated-1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mouse embryonic fibroblasts from wild type animals treated with H2O2
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E15
|
| Treatment protocol |
Cells were treated for one hour with 0.5 mM hydrogen peroxide and fed with fresh media without hydrogen peroxide before total RNA extraction 24hours later
|
| Growth protocol |
Cells were maintained in DMEM with 50 microg/ml streptomycin and 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sambrook et al., 1989. Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press.
|
| Label |
Cy5
|
| Label protocol |
Protocol from Agilent
|
| |
|
| Channel 2 |
| Source name |
Mouse embryonic fibroblasts from wild type animals
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E15
|
| Treatment protocol |
Cells were treated for one hour with 0.5 mM hydrogen peroxide and fed with fresh media without hydrogen peroxide before total RNA extraction 24hours later
|
| Growth protocol |
Cells were maintained in DMEM with 50 microg/ml streptomycin and 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sambrook et al., 1989. Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press.
|
| Label |
Cy3
|
| Label protocol |
Protocol from Agilent
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing was performed using the in situ Hybridization Plus kit (Agilent) and following the manufactures instructions.
|
| Scan protocol |
The arrays were scanned using a dual-laser DNA microarray scanner (Agilent). The data was then extracted from images by the Feature Extraction software 6.1 (Agilent).
|
| Description |
WT biological replicate 1 of 2.
|
| Data processing |
The data were background subtracted and normalized using the loess method all included in the limma package of BioConductor(www.bioconductor.org)
|
| |
|
| Submission date |
Nov 13, 2009 |
| Last update date |
Nov 13, 2009 |
| Contact name |
Eric R Paquet |
| Organization name |
EPFL
|
| Department |
Life sciences
|
| Lab |
Naef lab
|
| Street address |
Station 15 CH - 1015
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL7042 |
| Series (1) |
| GSE19007 |
H2O2 effect on mouse embryonic fibroblasts +/- a deletion in the helicase domain of the Werner Syndrome gene homologue |
|
