|
| Status |
Public on Jun 01, 2010 |
| Title |
AB34_WT_MMnit_3h_1 |
| Sample type |
RNA |
| |
|
| Source name |
U. maydis strain AB34 grown for 3h in array medium/nitrate
|
| Organism |
Mycosarcoma maydis |
| Characteristics |
time: 3h in array medium/nitrate
strain: AB34 is a FB2 (a2 b2) derivative in which the induction of the bE2 and bW2 genes is under control of the nitrate-inducible nar1 promoter.
|
| Biomaterial provider |
Mario Scherer, Chetsada Pothiratana and Jörg Kämper
|
| Growth protocol |
Strain AB34 was grown on a rotary shaker (200 rpm) to an OD600 of 0.4-0.6 at 28°C in liquid array medium: 6.25 % (w/v) salt solution, 30 mM L-glutamine, 1 % (w/v) glucose, pH 7.0 (filter-sterilized). bE2 and bW2 genes were induced for 3 hours in liquid array medium containing 3g/L nitrate instead of glutamine as a nitrogen source.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the TRIZOL-reagent (Invitrogen) according to the manufacturer's instructions. RNA samples were further column purified (Qiagen RNeasy) and the quality checked using a Bioanalyzer with a RNA 6000 Nano LabChip kit (Agilent).
|
| Label |
biotin/phycoerythrin
|
| Label protocol |
Target preparation was performed according to the standard Affymetrix protocol using the Enzo BioArray RNA transcript labelling kit, except for a reaction temperature of 50°C for first-strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
EukGE-WS2 protocol on a GeneChip Fluidics Station 400
|
| Scan protocol |
Agilent G2500A
|
| Description |
3h time point of nar1P controlled bE2/bW2 gene induction
|
| Data processing |
MAS5.1
|
| |
|
| Submission date |
Oct 27, 2009 |
| Last update date |
Nov 04, 2009 |
| Contact name |
Jörg Thomas Kämper |
| E-mail(s) |
joerg.kaemper@kit.edu
|
| Phone |
+49 721 608 5670
|
| Organization name |
Karlsruhe Institute of Technology
|
| Department |
Department for Applied Biosciences
|
| Street address |
Hertzstrasse 16, Geb. 06.40
|
| City |
Karlsruhe |
| ZIP/Postal code |
76187 |
| Country |
Germany |
| |
|
| Platform ID |
GPL3681 |
| Series (2) |
| GSE18750 |
Controlled expression of compatible and incompatible combinations of Ustilago maydis b-mating type locus genes bE and bW |
| GSE18881 |
Transcription factor Rbf1 is the master regulator for b-mating type controlled pathogenic development in Ustilago maydis |
|
