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Sample GSM464637 Query DataSets for GSM464637
Status Public on May 18, 2010
Title RER SCL no AB
Sample type SRA
Source name fetal liver erythroblasts
Organism Mus musculus
Characteristics cell type: fetal liver erythroblast
genotype: RER SCL mutant
Treatment protocol no treatments applied
Growth protocol mouse E12.5 fetal liver cells were expanded in vitro for 3 days in the presence of growth factors then ter119 purified. Ter119- populations were processed for ChIP experiments.
Extracted molecule genomic DNA
Extraction protocol Protein-DNA cross-linking was performed on 1 x 10^7 Ter119– cells with 1% formaldehyde for 10 min at room temperature. Glycine (0.125 M) was added to quench the reaction. Cells were washed twice in phosphate-buffered saline containing protease inhibitors (Complete; Roche). Nuclei were lysed in lysis buffer (ChIP assay kit; Upstate Biotechnology) and sheared by sonication to reduce the chromatin fragments to 200 to 500 base pairs. Immunoprecipitation of cross-linked chromatin was performed using anti-SCL and ready-made buffers (ChIP assay kit; Upstate Biotechnology). Control samples were treated exactly as the anti-SCL ChIP smaples except no antibody was added to the crosslinked and sonicated chromatin.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
Description ChIP enriched
Data processing wig files were made from maq pileup data and incorporated into GBrowse htt
Submission date Oct 23, 2009
Last update date May 15, 2019
Contact name Stephen Taylor
Phone +44 1865 222640
Organization name CBRG
Street address Headington
City Oxford
ZIP/Postal code OX3 9DS
Country United Kingdom
Platform ID GPL9185
Series (2)
GSE18720 Genome-wide mapping of in vivo SCL/DNA interactions in erythroid cells
GSE51338 Dynamic shifts in occupancy by TAL1 are guided by GATA factors and drive large-scale reprogramming of gene expression during hematopoiesis
SRA SRX018726
BioSample SAMN00010871

Supplementary file Size Download File type/resource
GSM464637_RER_SCL_no_AB.wig.gz 103.2 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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