|
| Status |
Public on May 18, 2010 |
| Title |
WT SCL |
| Sample type |
SRA |
| |
|
| Source name |
fetal liver erythroblasts
|
| Organism |
Mus musculus |
| Characteristics |
cell type: fetal liver erythroblast
genotype: wild type
|
| Treatment protocol |
no treatments applied
|
| Growth protocol |
mouse E12.5 fetal liver cells were expanded in vitro for 3 days in the presence of growth factors then ter119 purified. Ter119- populations were processed for ChIP experiments.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Protein-DNA cross-linking was performed on 1 x 10^7 Ter119– cells with 1% formaldehyde for 10 min at room temperature. Glycine (0.125 M) was added to quench the reaction. Cells were washed twice in phosphate-buffered saline containing protease inhibitors (Complete; Roche). Nuclei were lysed in lysis buffer (ChIP assay kit; Upstate Biotechnology) and sheared by sonication to reduce the chromatin fragments to 200 to 500 base pairs. Immunoprecipitation of cross-linked chromatin was performed using anti-SCL and ready-made buffers (ChIP assay kit; Upstate Biotechnology). Control samples were treated exactly as the anti-SCL ChIP smaples except no antibody was added to the crosslinked and sonicated chromatin.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
ChIP enriched
|
| Data processing |
wig files were made from maq pileup data and incorporated into GBrowse htt
|
| |
|
| Submission date |
Oct 23, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen Taylor |
| E-mail(s) |
stephen.taylor@imm.ox.ac.uk
|
| Phone |
+44 1865 222640
|
| Organization name |
CBRG
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL9185 |
| Series (2) |
| GSE18720 |
Genome-wide mapping of in vivo SCL/DNA interactions in erythroid cells |
| GSE51338 |
Dynamic shifts in occupancy by TAL1 are guided by GATA factors and drive large-scale reprogramming of gene expression during hematopoiesis |
|
| Relations |
| SRA |
SRX018723 |
| BioSample |
SAMN00010868 |
