|
| Status |
Public on Jun 28, 2021 |
| Title |
CWR22Rv1_-R_Input |
| Sample type |
SRA |
| |
|
| Source name |
CWR22Rv1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CWR22Rv1
chip antibody: none
|
| Treatment protocol |
For the control (+R) group, cells were maintained in the same culture medium for 48 h. For the arginine-deprived (-R) group, ADI-PEG20 (0.2 mg/ml) was added to deplete the arginine in the medium.
|
| Growth protocol |
All cells were maintained in RPMI 1640 supplemented with 100 U/ml penicillin/streptomycin and 10% FBS before experiments.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with formaldehye, and chromatin was extracted with Magna ChIP™ A/G (Millipore). Chromatin was sheared with a Bioruptor Plus sonicator and isolated with antibodies.
Libraries were prepared using a KAPA Hyper Prep Kit according to the instructions.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence and mapped to UCSC hg38 using BWA
Peak calling was performed using SICER.
Genome_build: UCSC hg38
Supplementary_files_format_and_content: bedgraph
|
| |
|
| Submission date |
Jun 04, 2020 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Sheng-Chieh Hsu |
| Organization name |
National Tsing Hua University
|
| Department |
Institute of Biotechnology
|
| Street address |
No. 101, Section 2, Kuang-Fu Road
|
| City |
Hsinchu |
| ZIP/Postal code |
30013 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE151809 |
Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes [ChIP-seq] |
| GSE151855 |
Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes. |
|
| Relations |
| BioSample |
SAMN15099925 |
| SRA |
SRX8470158 |
