|
| Status |
Public on Jun 28, 2021 |
| Title |
PC3_-R, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PC3 cultured in arginine-free medium for 48 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC3
treatment: arginine-free medium for 48 h
|
| Treatment protocol |
On the day of arginine deprivation experiments, culture medium was aspirated, and cells were rinsed with PBS once. Cells were culture in arginine-free RPMI supplied with 10% dialyzed FBS, 100 U/ml penicillin/streptomycin and 10 mM HEPES for indicated durations. For the control group, 200 mg/L L-arginine was added.
|
| Growth protocol |
All cells were maintained in RPMI 1640 supplemented with 100 U/ml penicillin/streptomycin and 10% FBS before experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNAspin Mini RNA Isolation Kit (GE Healthcare, 25-0500-72).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) Software.
|
| |
|
| Submission date |
Jun 03, 2020 |
| Last update date |
Jun 28, 2021 |
| Contact name |
Sheng-Chieh Hsu |
| Organization name |
National Tsing Hua University
|
| Department |
Institute of Biotechnology
|
| Street address |
No. 101, Section 2, Kuang-Fu Road
|
| City |
Hsinchu |
| ZIP/Postal code |
30013 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE151719 |
Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes [microarray] |
| GSE151855 |
Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes. |
|
