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Sample GSM4589058 Query DataSets for GSM4589058
Status Public on Jun 28, 2021
Title CWR22Rv1, -R0h
Sample type RNA
 
Source name CWR22Rv1 cultured in control medium for 24 h
Organism Homo sapiens
Characteristics cell line: CWR22Rv1
treatment: control medium for 24 h
Treatment protocol On the day of arginine deprivation experiments, culture medium was aspirated, and cells were rinsed with PBS once. Cells were culture in arginine-free RPMI supplied with 10% dialyzed FBS, 100 U/ml penicillin/streptomycin and 10 mM HEPES for indicated durations. For the control group, 200 mg/L L-arginine was added.
Growth protocol All cells were maintained in RPMI 1640 supplemented with 100 U/ml penicillin/streptomycin and 10% FBS before experiments.
Extracted molecule total RNA
Extraction protocol RNA was extracted with RNAspin Mini RNA Isolation Kit (GE Healthcare, 25-0500-72).
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) Software.
 
Submission date Jun 03, 2020
Last update date Jun 28, 2021
Contact name Sheng-Chieh Hsu
Organization name National Tsing Hua University
Department Institute of Biotechnology
Street address No. 101, Section 2, Kuang-Fu Road
City Hsinchu
ZIP/Postal code 30013
Country Taiwan
 
Platform ID GPL23159
Series (2)
GSE151719 Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes [microarray]
GSE151855 Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes.

Data table header descriptions
ID_REF
VALUE Quantification
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
AFFX-BkGr-GC03_st 6.99961 0.866441
AFFX-BkGr-GC04_st 7.00896 0.99793
AFFX-BkGr-GC05_st 6.82468 0.999984
AFFX-BkGr-GC06_st 6.91802 0.999997
AFFX-BkGr-GC07_st 6.40737 0.999993
AFFX-BkGr-GC08_st 3.46223 0.99999
AFFX-BkGr-GC09_st 3.09665 0.999886
AFFX-BkGr-GC10_st 2.83811 0.999779
AFFX-BkGr-GC11_st 2.75099 0.999177
AFFX-BkGr-GC12_st 2.64705 0.990735
AFFX-BkGr-GC13_st 2.50376 0.997557
AFFX-BkGr-GC14_st 2.34754 0.9923
AFFX-BkGr-GC15_st 2.34758 0.987345
AFFX-BkGr-GC16_st 2.40807 0.975305
AFFX-BkGr-GC17_st 2.4426 0.952622
AFFX-BkGr-GC18_st 2.65494 0.886533
AFFX-BkGr-GC19_st 5.40729 0.661525
AFFX-BkGr-GC20_st 5.49633 0.652556
AFFX-BkGr-GC21_st 5.56463 0.641795
AFFX-BkGr-GC22_st 5.58624 0.634538

Total number of rows: 24351

Table truncated, full table size 897 Kbytes.




Supplementary file Size Download File type/resource
GSM4589058_CWR22Rv1_0h.CEL.gz 1.0 Mb (ftp)(http) CEL
GSM4589058_CWR22Rv1_0h.chp.gz 270.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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