|
| Status |
Public on Nov 12, 2009 |
| Title |
HPB-ALL_SAHM1_03 |
| Sample type |
RNA |
| |
|
| Source name |
HPB-ALL cells treated with SAHM1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: T-cell
cell line: HPB-ALL
|
| Treatment protocol |
Cells were cultured in 24-well plates and indicated with SAHM1 or the equivalent amount of DMSO for 24 hours.
|
| Growth protocol |
T-ALL cells were grown in1640 RPMI with 10% FBS and 1% pen/strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Treated cells were centrifuged at 1000 rpm, washed with cold PBS and total RNA was extracted using a Qiagen Rneasy Kit according to manufacturers instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was generated using standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to standard protocols for Affymetrix HG-U133 plus 2.0 arrays.
|
| Scan protocol |
Arrays were scanned using a GenePix 4000B microarray scanner.
|
| Description |
none
|
| Data processing |
Data was processed using GenePattern software available at the Broad Insititute. RMA processing was used with quantile normalization.
|
| |
|
| Submission date |
Sep 21, 2009 |
| Last update date |
Oct 24, 2009 |
| Contact name |
Raymond Moellering |
| E-mail(s) |
rmoeller@fas.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
Chemistry and Chemical Biology
|
| Street address |
12 Oxford Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE18198 |
Expression profile of human T-ALL cell lines treated with DMSO or SAHM1 |
|
