|
| Status |
Public on May 20, 2020 |
| Title |
Monos31 |
| Sample type |
SRA |
| |
|
| Source name |
Peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
cells: Monocytes
treatment: Untreated
|
| Treatment protocol |
Isolated monocytes were cultured at 37 degree C for 16h in the presence or absence of dexamethasone (100ng/mL).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated from monocytes using Dynabeads mRNA isolation kit.
RNA-seq libraries were prepared using the NEBNext ultradirectional library preparation protocol (New England BioLabs, Ipswich, MA)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
RNA-seq data were analyzed using the Illumina Basespace RNA express application.
The sequencing reads were aligned to the reference human genome hg19 using STAR aligner and differentially expressed (DE) genes (FDR < 5%) were identified with the DEseq2 analysis tool.
|
| |
|
| Submission date |
May 07, 2020 |
| Last update date |
May 20, 2020 |
| Contact name |
Lobelia Samavati |
| E-mail(s) |
lsamavat@med.wayne.edu
|
| Phone |
3137451718
|
| Organization name |
Dr.
|
| Department |
Internal Medicine (H0609)
|
| Lab |
Center for Molecular Medicine
|
| Street address |
3990 John R#3 Hudson, Harper University Hospital
|
| City |
Detroit |
| State/province |
Michigan |
| ZIP/Postal code |
48202 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE150049 |
Trancriptional signatures of dexamethasone-treated sarcoidosis monocytes. |
|
| Relations |
| BioSample |
SAMN14848761 |
| SRA |
SRX8288057 |
