|
| Status |
Public on Jun 30, 2011 |
| Title |
6154 B10D2 -> balb rag2-/- (new) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
B10D2 -> balb rag2-/-
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin
|
| Treatment protocol |
Skin harvested from cGVHD mice at 2 weeks post splenocyte transfer.
|
| Growth protocol |
cGVHD mice generated by splenocyte transfer of B10.D2 into balb/c; syngeneic controls balb/c into balb/c.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated using Trizol methods; cleaned up with Qiagen RNeasy mini-columns.
|
| Label |
Cy3
|
| Label protocol |
Agilent Linear Amplification Low RNA Input Kit.
|
| |
|
| Channel 2 |
| Source name |
Universal Mouse Reference
|
| Organism |
Mus musculus |
| Characteristics |
cell line: multiple
|
| Treatment protocol |
Skin harvested from cGVHD mice at 2 weeks post splenocyte transfer.
|
| Growth protocol |
cGVHD mice generated by splenocyte transfer of B10.D2 into balb/c; syngeneic controls balb/c into balb/c.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated using Trizol methods; cleaned up with Qiagen RNeasy mini-columns.
|
| Label |
Cy5
|
| Label protocol |
Agilent Linear Amplification Low RNA Input Kit.
|
| |
|
| |
| Hybridization protocol |
Agilent Protocols and Hybridization Kits.
|
| Scan protocol |
GenePix 400B scanner (Axon Instruments).
|
| Description |
Universal Mouse Reference RNA (Stratagene).
|
| Data processing |
Feature extraction with GenePix 5.1 software.
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
Jun 30, 2011 |
| Contact name |
Jennifer L Sargent |
| E-mail(s) |
jennifer.sargent@gmail.com
|
| Organization name |
Dartmouth Medical School
|
| Department |
Genetics
|
| Lab |
Whitfield
|
| Street address |
Dartmouth College; HB7400
|
| City |
Hanover |
| State/province |
NH |
| ZIP/Postal code |
03755 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (2) |
| GSE17914 |
IL13 and CCL2 Drive Disease in an Inflammatory Subset of Scleroderma [cGVHD] |
| GSE24410 |
IL13 and CCL2 Drive Disease in an Inflammatory Subset of Scleroderma |
|
