|
| Status |
Public on Apr 10, 2024 |
| Title |
Clone2_Minus [RNA-Seq] |
| Sample type |
SRA |
| |
|
| Source name |
Mouse Bone Marrow ER-Hoxb8 monocytic cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: ER-Hoxb8 cells
|
| Growth protocol |
ER-HoxB8 cells were maintained in RPMI medium supplemented with 10% FBS, penicillin/streptomycin, and SCF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For RNA-seq, total RNAs were extracted using RNeasy mini kit (Qiagen).
RNA-sequencing libraries were generated using library prep kits (NEB) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Gene expression data in ER-Hoxb8 cells
|
| Data processing |
Illumina software used for basecalling.
Raw sequences of RNA-seq were mapped to the mouse transcript (mm10) using a Salmon mapper (v.1.1.0). The count of mapped reads was normalized as transcripts per million (TPM) using an R package of tximport.
Genome_build: mm10
Supplementary_files_format_and_content: TPM
|
| |
|
| Submission date |
Apr 13, 2020 |
| Last update date |
Apr 10, 2024 |
| Contact name |
David T Scadden |
| E-mail(s) |
dscadden@mgh.harvard.edu
|
| Organization name |
MGH
|
| Department |
Center for Regenerative Medicine
|
| Street address |
185 Cambridge St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE148554 |
Functionally distinct subset of monocytes in mouse and human blood [RNA-Seq] |
| GSE148555 |
Functionally distinct subset of monocytes in mouse and human blood |
|
| Relations |
| BioSample |
SAMN14591124 |
| SRA |
SRX8110292 |
