|
| Status |
Public on Nov 24, 2020 |
| Title |
BCPAP_siEPB41L4A_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
BCPAP cells treated with EPB41L4A siRNA for 72 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BCPAP
|
| Treatment protocol |
40 uM siEPB41L4A or siNS were transfected into BCPAP cells using 7.5 ul Lipofectamine RNAiMAX (Thermo Fisher Scientific). Cells were harvest after 72 hours of treatment.
|
| Growth protocol |
BCPAP cells were seeded in 6-well plate and grown to 75% confluence at the time of transfection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
5.5 ug of single-stranded cDNA were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized using herring sperm DNA, acetylated BSA (50 mg/mL). Eukaryotic Hybridization Controls (Affymetrix) were heated at 65°C for 5 minutes, 99°C for 5 minutes, 45°C for 5 minutes, centrifuged at maximum speed for 5 minutes, then hybridized 16 hours at 45°C at 60 rpm. Fluidics washing is protocol FS450_0001.
|
| Scan protocol |
The arrays were scanned with the Affymetrix Genen Chip Scannner 3000 7G system using Affymetrix GeneChip Command Console (AGCC) software.
|
| Data processing |
Signal intensities were processed by RMA method using Affymetrix Expression Console software
|
| |
|
| Submission date |
Apr 10, 2020 |
| Last update date |
Nov 24, 2020 |
| Contact name |
Albert de la Chapelle |
| E-mail(s) |
daniel.comiskey@osumc.edu
|
| Organization name |
The Ohio State University Wexner Medical Center
|
| Street address |
460 W. 12th Avenue
|
| City |
Columbus |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE148485 |
Expression data of BCPAP cells with EPB41L4A knockdown |
|
