|
| Status |
Public on Apr 05, 2020 |
| Title |
HCT116_water |
| Sample type |
genomic |
| |
|
| Source name |
HCT116
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
lig1_peptide: none
|
| Treatment protocol |
None
|
| Growth protocol |
37ºC, 5% CO2, McCoy's 5A media with 10% FBS and no antibiotics.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
3x extraction: phenol, phenol/chloroform/isoamyl alcohol (25:24:1), chloroform/isoamyl alcohol (24:1); precipitation; RNase treatment; precipitation.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina Protocol.
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to the Illumina Infinium MethylationEPIC BeadChip using standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using iScan using standard recommended Illumina scanner setting.
|
| Description |
water
|
| Data processing |
openSesame from SeSAMe R package.
|
| |
|
| Submission date |
Apr 04, 2020 |
| Last update date |
Jul 31, 2020 |
| Contact name |
Scott Rothbart |
| E-mail(s) |
scott.rothbart@vai.org
|
| Organization name |
Van Andel Research Institute
|
| Department |
Center for Epigenetics
|
| Lab |
Rothbart Lab
|
| Street address |
333 Bostwick Ave NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE148086 |
DNA methylation analysis of HCT116 after disruption of methyllysine reading and writing. |
|
