|
| Status |
Public on Apr 29, 2020 |
| Title |
21 days old ERa KO uterus E2, 2h-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
uterus from 21 day old ERa knockout mouse that was treated for 2h with E2
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Ex3aERKO
|
| Treatment protocol |
for saline vehicle: 100 ul of sailne injected intraperitoneally, tissue collected 24 hours later. For estradiol (E2) 250 ng of E2 dissolved in 100 ul of saline injected intraperitoneally, tissue collected after 2 hours or 24 hours. For IGF1 samples, 50 ug of IGF1 dissolved in PBS was injected intraperitoneally, and tissue collected after 2h, or 50 ug of IGF1 dissolved in PBS injected intraperitoneally 3 times (0, 8 and 16 hours after first injection), and tissue was collected 24h after the first injection
|
| Growth protocol |
pre-pubertal 21-days old female WT and ERa KO mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol reagent followed by Rneasy clean up protocol
|
| Label |
Biotin
|
| Label protocol |
Three hundred nanograms (300 ng) of total RNA were amplified and labeled as directed using the Affymetrix WT Plus Reagent Kit (WT Plus Kit).
|
| |
|
| Hybridization protocol |
Five and one half micrograms (5.5 μg) of amplified biotin-cDNAs were fragmented and hybridized to each array for 16 hours at 45°C in a rotating hybridization oven. Array slides were stained with streptavidin/phycoerythrin utilizing a double-antibody staining procedure and then washed for antibody amplification according to the GeneChip Hybridization, Wash and Stain Kit and user manual following protocol FS450-0001.
|
| Scan protocol |
Arrays were scanned in an Affymetrix Scanner 3000.
|
| Description |
Gene expression from uterus from 21 day old ERa knockout mouse that was treated for 2h with E2
|
| Data processing |
Data was obtained using the GeneChip® Command Console software.
|
| |
|
| Submission date |
Apr 02, 2020 |
| Last update date |
Apr 29, 2020 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE148006 |
Uterine transpriptome of pre-pubertal WT and ERa knockout (Ex3aERKO) mice |
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