|
| Status |
Public on Nov 16, 2020 |
| Title |
Non-tumoral gland A19-34 |
| Sample type |
RNA |
| |
|
| Source name |
Pituitary gland
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Non-tumoral gland
|
| Treatment protocol |
With no previous treatment
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip WT Pico Reagent Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Clariom D Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7G GeneChip scanner.
|
| Description |
Non-tumoral gland
Control-A19-34
Non-tumoral gland
|
| Data processing |
The data was analyzed with RMA using Partek Genomic Suite default analysis settings .
|
| |
|
| Submission date |
Mar 30, 2020 |
| Last update date |
Nov 16, 2020 |
| Contact name |
Daniel Marrero-Rodriguez |
| E-mail(s) |
dan.mar57@gmail.com
|
| Phone |
+5554401021
|
| Organization name |
Instituto Mexicano del Seguro Social
|
| Department |
Unidad de Investigacion Medica en Enfermedades Endocrinas
|
| Lab |
Laboratorio de Endocrinologia Experimental
|
| Street address |
Av. Cuauhtémoc 330, Col. Doctores
|
| City |
México City |
| State/province |
México City |
| ZIP/Postal code |
06720 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE147786 |
Transcriptome and methylome analysis reveal three cellular origins of pituitary tumors |
|
