|
| Status |
Public on Dec 31, 2009 |
| Title |
Whole oocyte rep2 |
| Sample type |
RNA |
| |
|
| Source name |
stage VI oocyte
|
| Organism |
Xenopus laevis |
| Characteristics |
developmental stage: full-grown stage VI oocyte
isolation: collagenase treatment
cell type: oocyte
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared by proteinase K digestion followed by phenol extraction and ethanol precipitation. Purified RNA was treated with DNase prior to cDNA synthesis. RNA quality was verified using the Experion System (BioRad) for automated electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
100 ng of total RNA was used to make complementary RNA (cRNA)d, which was amplified using the GeneChip® Two-Cycle cDNA Synthesis Kit (Invitrogen/Affymetrix). cRNA labeling, hybridization and data acquisition were performed by The University of Iowa, Carver College of Medicine DNA Core Facility, using standard Affymetrix protocols and reagents.
|
| |
|
| Hybridization protocol |
Labelled cRNA was hybridized to Xenopus laevis Genome Arrays (Affymetrix) at 45˚C using Model 640 hyb ovens, processed using the Model 450 GeneChip fluidics station (Affymetrix).
|
| Scan protocol |
Image files were captured on a Model 3000 GeneArray scanner and processed using Affymetrix micorarray analysis software.
|
| Description |
Sample Number: 2
Replicate Number: 2
Experimental condition: Control
|
| Data processing |
Quality control included standard Affymetrix internal controls. GeneChip Operating software was used to generate data files (CEL), and MAS 5 for calculating expression signals.
|
| |
|
| Submission date |
Aug 18, 2009 |
| Last update date |
Aug 19, 2009 |
| Contact name |
Douglas W. Houston |
| E-mail(s) |
douglas-houston@uiowa.edu
|
| Phone |
(319) 335-1316
|
| Organization name |
The University of Iowa
|
| Department |
Department of Biology
|
| Street address |
257 BB
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE17713 |
Microarray analysis of mRNAs enriched in the vegetal cortex of Xenopus oocytes |
|
