|
| Status |
Public on Dec 01, 2009 |
| Title |
Cord Blood 19, biological replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Reticulocytes from umbilical cord blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: umbilical cord blood
cell type: reticulocytes
|
| Treatment protocol |
Reticulocytes-enriched pools were prepared from blood samples by removing plasma, platelets and nucleated cells using centrifugation and filtration methods.
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 7G 3000.
|
| Description |
Gene expression data of reticulocytes from umbilical cord blood.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Aug 13, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jeffery Lynn Miller |
| E-mail(s) |
jm7f@nih.gov
|
| Phone |
301-480-1908
|
| Fax |
301-435-5148
|
| Organization name |
NIH/NIDDK
|
| Department |
Molecular Medicine Branch
|
| Lab |
Molecular Biology and Genetics Section
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE17639 |
The human reticulocyte transcriptome (HG-U133_Plus2.0) |
|
| Relations |
| Reanalyzed by |
GSE119087 |
