|
| Status |
Public on Feb 01, 2010 |
| Title |
Sorted Lgr5 high vs. low cells colour switch 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
sorted pyloric stomach cells expressing Lgr5 at a high level
|
| Organism |
Mus musculus |
| Characteristics |
mouse model: Lgr5-EGFP-ires-CreERT2 knock-in mice
tissue: pyloric stomach
|
| Treatment protocol |
For the pyloric stem cell signature we used cells from Lgr5-EGFP-ires-CreERT2 knock-in mice, expressing GFP under the control of the Lgr5 promoter. RNA was isolated from two FACS sorted cell populations, one expressing GFP at high levels (GFPhigh) and the other expressing GFP at low levels (GFPlow).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen)
|
| Label |
Cy5
|
| Label protocol |
150 ng of total RNA was labeled using low RNA Input Linear Amp kit (Agilent Technologies, Pato Alto, CA, USA).
|
| |
|
| Channel 2 |
| Source name |
sorted pyloric stomach cells expressing Lgr5 at a low level
|
| Organism |
Mus musculus |
| Characteristics |
mouse model: Lgr5-EGFP-ires-CreERT2 knock-in mice
tissue: pyloric stomach
|
| Treatment protocol |
For the pyloric stem cell signature we used cells from Lgr5-EGFP-ires-CreERT2 knock-in mice, expressing GFP under the control of the Lgr5 promoter. RNA was isolated from two FACS sorted cell populations, one expressing GFP at high levels (GFPhigh) and the other expressing GFP at low levels (GFPlow).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
150 ng of total RNA was labeled using low RNA Input Linear Amp kit (Agilent Technologies, Pato Alto, CA, USA).
|
| |
|
| |
| Hybridization protocol |
according to Agilent guidelines
|
| Scan protocol |
according to Agilent guidelines
|
| Description |
n/a
|
| Data processing |
Microarray signal, background information, and default 2 channel normalization were performed using Feature Extraction (V.9.5.3, Agilent Technologies).
|
| |
|
| Submission date |
Aug 03, 2009 |
| Last update date |
Apr 01, 2010 |
| Contact name |
Daniel E. Stange |
| E-mail(s) |
daniel.stange@uniklinikum-dresden.de
|
| Organization name |
University Clinic Dresden
|
| Street address |
Fetscherstr. 74
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE17485 |
Active Lgr5 Positive Stem Cells Drive Self-Renewal in the Pyloric Stomach and Efficiently Build Pyloric Units In-vitro |
|
