|
| Status |
Public on Jul 31, 2009 |
| Title |
Stmn1-WT v Stmn1-null Array 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA extraction from tissue culture of stmn1 +/+ embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: wild type
cell type: embryonic fibroblasts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Used Trizol Extration from cultured cells followed by BioChip quality check, DNase treatment, quantification of RNA.
|
| Label |
Cy5
|
| Label protocol |
Followed Agilent protocol for linear amplification of cDNA sample labeling.
|
| |
|
| Channel 2 |
| Source name |
Total RNA extraction from tissue culture of stmn1 -/- embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: stmn1 -/-
cell type: embryonic fibroblasts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Used Trizol Extration from cultured cells followed by BioChip quality check, DNase treatment, quantification of RNA.
|
| Label |
Cy3
|
| Label protocol |
Followed Agilent protocol for linear amplification of cDNA sample labeling.
|
| |
|
| |
| Hybridization protocol |
default Agilent protocol
|
| Scan protocol |
default Agilent protocol
|
| Description |
n/a
|
| Data processing |
default Agilent protocol
|
| |
|
| Submission date |
Jul 30, 2009 |
| Last update date |
Jul 30, 2009 |
| Contact name |
Danielle N. Ringhoff |
| E-mail(s) |
dnr204@lehigh.edu
|
| Organization name |
Lehigh University
|
| Department |
Biological Sciences
|
| Lab |
Cassimeris
|
| Street address |
111 Research Drive
|
| City |
Bethlehem |
| State/province |
PA |
| ZIP/Postal code |
18015 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE17432 |
Murine Embryonic Fibroblasts: Wild-type Stmn1 versus null Stmn1 Cells |
|
