|
| Status |
Public on Feb 04, 2020 |
| Title |
MO6: kera_diff_donor1 |
| Sample type |
genomic |
| |
|
| Source name |
primary keratinocytes, fully differentiated status
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skin
cell type: primary keratinocytes
|
| Treatment protocol |
primary keratinocytes were cultured to 50% confluency (proliferation status) or allowed to full differentiate (fully differentiated status)
|
| Growth protocol |
primary keratinocytes were cultured in KGM +growth factors untill ~50% confluency (proliferation status) or allowed to reach 100% confluency, switch to KGM lacking growth factors, and allowed to differentiate for an additional 6 days
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted using the QIAamp DNA blood mini kit according to the manufacturers protocol
|
| Label |
none
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
rnbeads, methylumi norm; Limma M value differential expression
|
| |
|
| Submission date |
Feb 03, 2020 |
| Last update date |
Feb 04, 2020 |
| Contact name |
Jos P.H. Smits |
| E-mail(s) |
jos.ph.smits@radboudumc.nl
|
| Organization name |
Radboudumc
|
| Department |
Dermatology
|
| Street address |
Rene Descartesdreef 1
|
| City |
Nijmegen |
| State/province |
Gelderland |
| ZIP/Postal code |
6525GL |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE144669 |
DNA methylation profiling of proliferating and differentiating keratinocytes |
|
