|
| Status |
Public on Feb 29, 2020 |
| Title |
Growth plate_Hypertrophic Zone_4 |
| Sample type |
RNA |
| |
|
| Source name |
Growth plate Hypertrophic Zone
|
| Organism |
Mus musculus |
| Characteristics |
tissue: femoral growth plate cartilage
zone: Hypertrophic Zone
strain: Swiss White
age: 14 days-old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure RNA isolation kit (Arcturus Bioscience, Mountain View, CA)
|
| Label |
Cy5
|
| Label protocol |
Cy3 and Cy5 dyes (Amersham Biosciences, Piscataway, NJ)
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit to 44K whole-genome microarrays (G4122A, Agilent Technologies, Santa Clara, CA
|
| Scan protocol |
Agilent scanner G2505B
|
| Data processing |
Agilent Feature Extraction V8.1.1.1
Threshold: 1.0
Logbase: 2
Technology: Agilent.SingleColor.12694
Normalization: Shift to 75 percentile
Baseline Transformation: None
|
| |
|
| Submission date |
Jan 28, 2020 |
| Last update date |
Mar 01, 2020 |
| Contact name |
Bent Brachvogel |
| E-mail(s) |
bent.brachvogel@uni-koeln.de
|
| Organization name |
University of Cologne
|
| Street address |
Joseph-Stelzmann Street 52
|
| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7042 |
| Series (1) |
| GSE144362 |
Sorting of Growth Plate Chondrocytes Allows the Isolation and Characterization of Cells of a Defined Differentiation Status |
|
