|
| Status |
Public on Apr 27, 2021 |
| Title |
Primary_IPF_cell_line_4 |
| Sample type |
RNA |
| |
|
| Source name |
Primary pulmonary fibroblasts from Idiopathic pulmonary fibrosis patient 4
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Primary pulmonary fibroblasts
age: 68
Sex: Male
smoking status: Ex-smoker
|
| Treatment protocol |
No additional treatments were applied to the cultured cell lines.
|
| Growth protocol |
Cells were plated at a density of 2000 cells/cm2 and grown for 72 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen Rneasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with 3'IVT Express Kit and Affymetrix GeneChip® Hybridization, Wash and Stain Kit.
|
| |
|
| Hybridization protocol |
GeneChips were washed and stained in the Affymetrix GeneChip® Fluidics Station 450; fluidics script: FS450_0001.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 7G.
|
| Description |
Gene expression data from stromal cells cultured from IPF lung tissue
|
| Data processing |
Analysis of the microarray expression data was done using the R/Bioconductor through a graphical user interface, Chipster 3.12.5. Normalization was performed using custom chip description file for hgu133Aplus2. Intensity data were log2-transformed, and quantile normalized using robust multi-array average (RMA).
|
| |
|
| Submission date |
Jan 27, 2020 |
| Last update date |
May 01, 2021 |
| Contact name |
Mervi Kreus |
| Organization name |
University of Oulu
|
| Street address |
Aapistie 5A
|
| City |
Oulu |
| ZIP/Postal code |
90220 |
| Country |
Finland |
| |
|
| Platform ID |
GPL22321 |
| Series (1) |
| GSE144338 |
Expression data from stromal cells derived from idiopathic pulmonary fibrosis, lung adenocarcinoma and normal lung. |
|
