|
| Status |
Public on Jul 10, 2009 |
| Title |
Recovered Ro1 mouse cardiac ventricle, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Recovered Ro1 mouse cardiac ventricle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: transgenic mouse heart, expressing the g alpha i coupled receptor, ro1 (rassl or receptor activated solely by a synthetic ligand type opioid 1) protein
|
| Treatment protocol |
transgenic mouse heart, expressing the g alpha i coupled receptor, ro1 (rassl or receptor activated solely by a synthetic ligand type opioid 1) protein under the conditional control of the myosin-alpha-heavy chain promoter using the tetracycline transactivator (tta) system. cardiac ventrical was harvested from fvbn\ mice withdrawn from a doxycycline chow diet for 8-weeks (developing a severe dilated cardiomyopathy) and then recovered for 4-weeks by re-administration of doxycyline. mice were raised (fetal through adulthood) on doxycycline (redfern ch et al. proc natl acad sci u s a. 2000 apr 25;97(9): 4826-31).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was converted to cDNA primed with random hexamers using the SuperScript II enzyme (Invitrogen), fragmented with DNAse1 (0.6U/ul) (Amersham Pharmacia Biotech) at 37 C to yield 50-200nt fragements (Agilent Bioanalyzer), purifie
|
| |
|
| Hybridization protocol |
According to manufacturer's (Affymetrix) recommendations
|
| Scan protocol |
According to manufacturer's (Affymetrix) recommendations
|
| Description |
Exon-junction, exon, constitutive region hybridizing probesets from undifferentiated wild-type E14 embryonic stem cells
|
| Data processing |
Probeset intensity values for Affymetrix AltMouseA Array(perfect match only custom array) CEL files were obtained using the RMA package from Bioconductor, using the appropriate CDF file
|
| |
|
| Submission date |
Jul 08, 2009 |
| Last update date |
Jul 09, 2009 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Biomedical Informatics
|
| Lab |
Nathan Salomonis
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL8815 |
| Series (1) |
| GSE17021 |
Analysis of alternative splicing in mouse tissues with differentiation, disease and development. |
|
