|
| Status |
Public on Jan 09, 2020 |
| Title |
dorsal_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Brainstem_dorsal
|
| Organism |
Mus musculus |
| Characteristics |
strain: Chat-Cre: Ai9
Sex: male
age: 8 weeks
tissue: Brainstem; dorsal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Following tissue lysis using QIAzol Lysis Reagent (Qiagen), total RNA was extracted and purified using miRNeasy Micro Kit (Qiagen) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with lumi
|
| |
|
| Submission date |
Jan 08, 2020 |
| Last update date |
Jan 09, 2020 |
| Contact name |
Yu Hayashi |
| E-mail(s) |
hayashi.yu.fp@u.tsukuba.ac.jp
|
| Phone |
+29-853-3852
|
| Organization name |
University of Tsukuba
|
| Street address |
Tennodai 1-1-1
|
| City |
Tsukuba-shi |
| ZIP/Postal code |
305-8575 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21278 |
| Series (1) |
| GSE143280 |
Widely distributed neurotensinergic neurons in the brainstem regulate NREM sleep in mice |
|
