|
| Status |
Public on Sep 30, 2009 |
| Title |
SG200dhxt1 GM 1% Glucose 2 |
| Sample type |
RNA |
| |
|
| Source name |
U. maydis strain SG200-deltahxt1 grown in 1% glucose for 6 hours to an OD600 of 1
|
| Organism |
Mycosarcoma maydis |
| Characteristics |
strain: SG200-deltahxt1 is a SG200-derived strain with a deletion of the hxt1 gene (um05023 replaced by hygromycin).
|
| Biomaterial provider |
Ramon Wahl and Jörg Kämper
|
| Growth protocol |
Pre-cultures of SG200-deltahxt1 were grown overnight in liquid array medium (6.25 % (w/v) Holiday salt solution, 30 mM L-glutamine, pH 7.0, filter sterilized) containing 1% glucose (w/v). SG200-deltahxt1 cells were shifted to fresh liquid array medium containing 1% glucose and incubated for 6 hours to an OD600 of 1.0. RNA samples were frozen in liquid nitrogen for two independently conducted replicates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the TRIZOL-reagent (Invitrogen) according to the manufacturer`s instructions. RNA samples were further column purified (Qiagen RNeasy) and the quality checked using a Bioanalyzer with a RNA 6000 Nano LabChip kit (Agilent).
|
| Label |
biotin/phycoerythrin
|
| Label protocol |
Target preparation was performed according to the standard Affymetrix protocol using the Enzo BioArray RNA transcript labeling kit, exept for a reaction temperature of 50°C for first-strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
Affymetrix EukGe2V4 protocol on GeneChip Fluidics Station 400
|
| Scan protocol |
Affymetrix GSC3000
|
| Description |
Experiment for measuring the effects of the deletion of the sugar transporter gene hxt1 in SG200 during growth on glucose.
|
| Data processing |
MAS5.1
|
| |
|
| Submission date |
Jun 18, 2009 |
| Last update date |
Jun 19, 2009 |
| Contact name |
Jörg Thomas Kämper |
| E-mail(s) |
joerg.kaemper@kit.edu
|
| Phone |
+49 721 608 5670
|
| Organization name |
Karlsruhe Institute of Technology
|
| Department |
Department for Applied Biosciences
|
| Street address |
Hertzstrasse 16, Geb. 06.40
|
| City |
Karlsruhe |
| ZIP/Postal code |
76187 |
| Country |
Germany |
| |
|
| Platform ID |
GPL3681 |
| Series (1) |
| GSE16634 |
Gene expression on different carbon sources in response to hxt1 deletion in Ustilago maydis |
|
