 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 06, 2020 |
| Title |
w1118_Female_2hr_2 |
| Sample type |
SRA |
| |
|
| Source name |
Brain
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: w1118
Sex: Female
treatment: traumatic brain injury
time point: 2hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Direct-zolä RNA MicroPrep kit
QuantSeq 3' mRNA-Seq Library Prep Kit FWD (low-input protocol and 15 ng RNA input)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
After de-multiplexing with Illumina’s CASAVA 1.8.2 software, the 50 bp reads were aligned to the Drosophila genome (Build dm3) with STAR_2.4 and tabulated for each gene region.
Differential gene expression analysis was used to compare transcriptome changes between conditions using edgeR v.3.22.3 and transcripts were defined as significantly differentially expressed at absolute Log2 fold change >1, FDR <0.05
Genome_build: Dm3
Supplementary_files_format_and_content: Differential gene expression and counts data.xlsx
|
| |
|
| Submission date |
Nov 19, 2019 |
| Last update date |
Jul 06, 2020 |
| Contact name |
Ekta J Shah |
| E-mail(s) |
shahekta14@gmail.com
|
| Organization name |
Wayne State University
|
| Street address |
275 E. Hancock
|
| City |
Detroit |
| State/province |
Michigan |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL25244 |
| Series (1) |
| GSE140663 |
Drosophila Exhibit Divergent Sex-Based Responses in Transcription and Motor Function After Traumatic Brain Injury |
|
| Relations |
| BioSample |
SAMN13327452 |
| SRA |
SRX7182873 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
