|
| Status |
Public on Oct 01, 2022 |
| Title |
2dpi-L1 |
| Sample type |
SRA |
| |
|
| Source name |
leaves
|
| Organism |
Solanum lycopersicum |
| Characteristics |
infection: 2dpi
tissue: leaves
cultivar: Heinz 1706
|
| Treatment protocol |
The detached tomato leaves were inoculated with four 20 µl (200 zoospores per microliter) droplets of the zoospore solution
|
| Growth protocol |
Solanum lycopersicum (Heinz 1706) plants were grown in a greenhouse for 8-10 weeks under a 14 h day at 24°C and 10 h night at 22°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted using the Invitrogen PureLink RNA Mini kit (Cat no. 12183018A)
These libraries were sequenced using Illumina HiSeq 2X150 in paired-end mode with a read length of 150 bp.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
infection sample-2dpi
|
| Data processing |
The RNA-seq reads were aligned to the tomato genome (ITAG2.4) using tophat-2.0.11 with anchor length more than 8nt for spliced alignments. Only uniquely mapped reads were retained for subsequent analysis.
Genome_build: ITAG2.4
Supplementary_files_format_and_content: bigWig
|
| |
|
| Submission date |
Nov 18, 2019 |
| Last update date |
Oct 01, 2022 |
| Contact name |
jie Huang |
| E-mail(s) |
jiehuang@njau.edu.cn
|
| Organization name |
NJAU
|
| Street address |
NO.1 Weigang
|
| City |
Nanjing |
| ZIP/Postal code |
210095 |
| Country |
China |
| |
|
| Platform ID |
GPL19694 |
| Series (1) |
| GSE140537 |
RNA-seq data of Phytophthora infestans 88069 infected tomato leaves |
|
| Relations |
| BioSample |
SAMN13319772 |
| SRA |
SRX7175147 |
