|
Status |
Public on Oct 01, 2022 |
Title |
1dpi-L2 |
Sample type |
SRA |
|
|
Source name |
leaves
|
Organism |
Solanum lycopersicum |
Characteristics |
infection: 1dpi tissue: leaves cultivar: Heinz 1706
|
Treatment protocol |
The detached tomato leaves were inoculated with four 20 µl (200 zoospores per microliter) droplets of the zoospore solution
|
Growth protocol |
Solanum lycopersicum (Heinz 1706) plants were grown in a greenhouse for 8-10 weeks under a 14 h day at 24°C and 10 h night at 22°C
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was extracted using the Invitrogen PureLink RNA Mini kit (Cat no. 12183018A) These libraries were sequenced using Illumina HiSeq 2X150 in paired-end mode with a read length of 150 bp.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
infection sample-1dpi
|
Data processing |
The RNA-seq reads were aligned to the tomato genome (ITAG2.4) using tophat-2.0.11 with anchor length more than 8nt for spliced alignments. Only uniquely mapped reads were retained for subsequent analysis. Genome_build: ITAG2.4 Supplementary_files_format_and_content: bigWig
|
|
|
Submission date |
Nov 18, 2019 |
Last update date |
Oct 01, 2022 |
Contact name |
jie Huang |
E-mail(s) |
jiehuang@njau.edu.cn
|
Organization name |
NJAU
|
Street address |
NO.1 Weigang
|
City |
Nanjing |
ZIP/Postal code |
210095 |
Country |
China |
|
|
Platform ID |
GPL19694 |
Series (1) |
GSE140537 |
RNA-seq data of Phytophthora infestans 88069 infected tomato leaves |
|
Relations |
BioSample |
SAMN13319731 |
SRA |
SRX7175144 |