|
| Status |
Public on Oct 31, 2019 |
| Title |
GOLNL7dR2 |
| Sample type |
SRA |
| |
|
| Source name |
tomato leaf
|
| Organism |
Solanum lycopersicum |
| Characteristics |
time: 7 day
treatment: Low Nitrogen
cultivar: Guozhen No.1
tissue: leaf
|
| Treatment protocol |
The plants were grown under hydroponic conditions in a growth chamber, and were grown in full-strength Hoagland solution containing normal N (9 mM) or low N (1 mM)
|
| Growth protocol |
During the culture, the plants were grown under light conditions at an average photosynthetic photon-flux density of 300 μmol m-2·s-1. The day and night temperatures of the growth chamber were 28°C and 18°C, respectively, and its day relative humidity was 75%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA prep Pure Plant Kit (No. DP441; Tiangen Co. Ltd, China)
NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Quality control
Comparative analysis: Hisat2 tools soft were used to map with reference genome.
The gene expression analyses were performed with StringTie
Supplementary_files_format_and_content: tab-delimited list file includes FPKM values for each Sample and infromatiom of new genes...
|
| |
|
| Submission date |
Oct 25, 2019 |
| Last update date |
Oct 31, 2019 |
| Contact name |
Jianda Shi |
| E-mail(s) |
kingdacommit@gmail.com
|
| Phone |
17607100847
|
| Organization name |
HZAU
|
| Street address |
Hube
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430000 |
| Country |
China |
| |
|
| Platform ID |
GPL24124 |
| Series (1) |
| GSE139405 |
RNA-seq of two contrasting tomato genotypes in response to low nitrogen |
|
| Relations |
| SRA |
SRX7032167 |
| BioSample |
SAMN13073510 |
