|
| Status |
Public on Aug 13, 2009 |
| Title |
E14 clone with pSicoR-mCherry Chd1 shRNAi4 C4i2 |
| Sample type |
RNA |
| |
|
| Source name |
ES cells, Chd1-deficient
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E14 clone C4i2
|
| Treatment protocol |
Cells trypsinized with 0.05% Trypsin for 3min at 37C and resuspended in PBS with trypsin inactivator.
|
| Growth protocol |
Cells on gelatin-coated plates with ES cell medium with 15% KSR and recombinant LIF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
According to manufacturer's instructions at the Genomics Core Facility of the Gladstones Institutes.
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions at the Genomics Core Facility of the Gladstones Institutes.
|
| Scan protocol |
According to manufacturer's instructions at the Genomics Core Facility of the Gladstones Institutes.
|
| Description |
Gene expression data of ES cells grown in normal ES cell conditions.
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jun 05, 2009 |
| Last update date |
Aug 10, 2009 |
| Contact name |
Miguel Ramalho-Santos |
| E-mail(s) |
mrsantos@diabetes.ucsf.edu
|
| Organization name |
UCSF
|
| Department |
Eli and Edythe Broad Center of Regeneration Medicine
|
| Lab |
Santos lab
|
| Street address |
35 Medical Center Way
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE16462 |
Expression data from Chd1-deficient mouse ES cells (E14 cell lines) and genome-wide binding of Chd1 in parental ES cells |
|
