|
| Status |
Public on Jan 26, 2010 |
| Title |
XXX_5490 |
| Sample type |
RNA |
| |
|
| Source name |
XXX_5490
|
| Organism |
Homo sapiens |
| Characteristics |
agebin: 1
t: T2
n: N0
grade: 3
her2fish: 0.95
her2fishbin: 0
top2atri: -1
topoihc: 2
esr1bimod: 0
erbb2bimod: 0
final_analysis: YES
pcr: 1
dmfs_event: 0
dmfs_time: 1998
os_event: 0
os_time: 1998
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturers instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc).
|
| Label |
biotin
|
| Label protocol |
Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures.
|
| Scan protocol |
standard Affymetrix procedures.
|
| Description |
Please see the GSE16446_TOP_README.txt supplementary file on the Series record for a definition of the clinical characteristics
|
| Data processing |
RMA Bioconductor (default parameters) and log2 transformation.
|
| |
|
| Submission date |
Jun 02, 2009 |
| Last update date |
Jun 06, 2022 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
benjamin.haibe.kains@utoronto.ca
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16446 |
Multifactorial Approach to Predicting Resistance to Anthracyclines |
|
| Relations |
| Reanalyzed by |
GSE205568 |
