|
| Status |
Public on Sep 29, 2020 |
| Title |
CSF_BM_54 |
| Sample type |
RNA |
| |
|
| Source name |
CSF from Melanoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Cerebrospinal fluids
age: 42
|
| Treatment protocol |
All LMC samples are pretreatment, but BM & BT are mixed in terms of treatment status.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
RNA was labeled with an Affymetrix Flash Tag Biotin HSR RNA Labeling Kit according to standard procedure.
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to Affymetrix GeneChip microRNA 4.0 arrays and run using a Fluidics Station 450 protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
microRNA expression
|
| Data processing |
The data were analyzed with RMA-DMBG as normalization method using Affymetrix Expression Console default analysis settings.
|
| |
|
| Submission date |
Sep 27, 2019 |
| Last update date |
Sep 30, 2020 |
| Contact name |
Ho-Shin Gwak |
| E-mail(s) |
nsghs@kcch.re.kr
|
| Phone |
+82-10-7451-0413
|
| Organization name |
National Cancer Center Graduate School of Cancer Science and Policy
|
| Department |
Dept. of Cancer Control
|
| Street address |
323 Ilsan-ro, Ilsandong-gu
|
| City |
Goyang |
| State/province |
Non US |
| ZIP/Postal code |
10408 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE138092 |
microRNA expression Data from cerebrospinal fluid of cancer patients |
|
