|
| Status |
Public on Sep 20, 2019 |
| Title |
persister cell polysome pools_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
A375 persister cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: melanoma
|
| Treatment protocol |
One day after spliting A375 cells were treated for 3 days with DMSO or Vemurafenib and combimetinib
|
| Growth protocol |
A375 melanoma cells were cultured according to the ATCC protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and polysomal associated RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Accoding to Affymetrix protocol
|
| |
|
| Hybridization protocol |
Accoding to Affymetrix protocol
|
| Scan protocol |
Accoding to Affymetrix protocol
|
| Description |
polysome RNA
A375 persister cell on Day 1
|
| Data processing |
The data were analyzed with Affymetrix default analysis settings and global scaling as normalization method.
Clariom_D_Human.r1.pgf
Clariom_D_Human.na36.hg38.transcript.csv
|
| |
|
| Submission date |
Sep 19, 2019 |
| Last update date |
Sep 20, 2019 |
| Contact name |
Shensi SHEN |
| E-mail(s) |
shensi.shen@gustaveroussy.fr
|
| Organization name |
Gustave Roussy Institute
|
| Street address |
114 Rue Edouard Vaillant
|
| City |
Villejuif |
| ZIP/Postal code |
94805 |
| Country |
France |
| |
|
| Platform ID |
GPL23126 |
| Series (2) |
| GSE137724 |
Expression data from A375 melanoma parental and persister cells |
| GSE137726 |
A375 melanoma parental and persister cells |
|
