|
Status |
Public on Nov 25, 2020 |
Title |
MSC.MGUS2 |
Sample type |
genomic |
|
|
Source name |
Bone marrow
|
Organism |
Homo sapiens |
Characteristics |
Sex: M age: 61 donor: MGUS batch: batch1
|
Treatment protocol |
MSCs were recovered by trypsinization, washed once in PBS and stored at -20°C in DNA extraction buffer
|
Growth protocol |
Primary MSCs from bone marrow (BM) samples of healthy donors (n=8), MGUS (n=10) and SMM (n=8), multiple myeloma (MM) patients (n=9) were generated as described by Garayoa et al. 2009 (PMID:19357701). Ficoll-Paque density gradient separation medium was used to isolate mononuclear cells from BM aspirates. MSCs were selected by adherence to plastic and subsequently expanded until passage 2 when they were used for experiments.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted using proteinase K according to standard protocols
|
Label |
Cy3, Cy5
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium MethylationEPIC Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
Description |
200325500026_R08C01 DNA methylation data from human clinical samples
|
Data processing |
Rstudio
|
|
|
Submission date |
Sep 13, 2019 |
Last update date |
Nov 25, 2020 |
Contact name |
Esteban Ballestar |
Organization name |
Josep Carreras Research Institute (IJC)
|
Lab |
Epigenetics and Immune Disease
|
Street address |
Ctra de Can Ruti, Camí de les Escoles s/n
|
City |
Badalona, Barcelona |
State/province |
N/A = Not Applicable |
ZIP/Postal code |
08916 |
Country |
Spain |
|
|
Platform ID |
GPL21145 |
Series (2) |
GSE137416 |
Targeting aberrant DNA methylation in mesenchymal stromal cells as a treatment for myeloma bone disease [methylation II] |
GSE137419 |
Targeting aberrant DNA methylation in mesenchymal stromal cells as a treatment for myeloma bone disease |
|