|
| Status |
Public on Nov 03, 2021 |
| Title |
High fat diet_no bacteria_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Liver High fat diet_no bacteria_rep3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6N/Jcl
age: 10 weeks old
gender: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from liver samples was extracted using a RNeasy Mini kit, treated with DNase I (Qiagen, Germantown, MD).
|
| Label |
Cy3
|
| Label protocol |
Labeled cRNA were prepared from 1~5 ug total RNA using the Agilent’s Quick Amp Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.65 ug of cRNA were hybridized to the Agilent expression microarray according to the protocols provided by the manufacturer
|
| Scan protocol |
Arrays were scanned using the Agilent Technologies G4900DA SG12494263
|
| Data processing |
Array data export processing and analysis was performed using Agilent Feature Extraction v11.0.1.1
|
| |
|
| Submission date |
Sep 05, 2019 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Kazuhisa Yamazaki |
| E-mail(s) |
kaz@dent.niigata-u.ac.jp
|
| Organization name |
Niigata University
|
| Street address |
2-110-1 Gakkocho-dori, Chu-o-ku
|
| City |
Niigata |
| ZIP/Postal code |
951-8514 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21163 |
| Series (1) |
| GSE136937 |
Changes in gut microbiota induced by oral pathobiont deteriorate the pathology of non-alcoholic fatty liver disease |
|
