|
| Status |
Public on Feb 25, 2020 |
| Title |
ESCs_no treat with control siRNA_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
non treated-endometrial stromal cells with control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: human endometrium
gender: female
|
| Treatment protocol |
ESCs were transfected with WT1 siRNA and then treated with or without dibutyryl cyclic adenosine monophosphate (cAMP), a decidualization stimulus, for 4 days to induce decidualization
|
| Growth protocol |
ESCs were incubated in Phenol Red-free DMEM containing glutamine, antibiotics, and 10% dextran-coated charcoal-stripped FBS at 37°C, 95% air and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the cultured cells with an RNeasy® Mini Kit.
|
| Label |
biotin
|
| Label protocol |
The cRNA was amplified, labeled using GeneChip WT PLUS Reagent Kit according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to Affymetrix Clariom D Assay human array, washed and stained according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Affymetrix scanner.
|
| Description |
no treatment with control siRNA
|
| Data processing |
The data were analyzed with median polish summarization and quantile normalization by Affymetrix® Expression Console™ 1.1 software, and obtained normalized intensity values.
|
| |
|
| Submission date |
Aug 29, 2019 |
| Last update date |
Feb 26, 2020 |
| Contact name |
Isao Tamura |
| E-mail(s) |
isao@yamaguchi-u.ac.jp
|
| Phone |
+81 836 22 2289
|
| Organization name |
Yamaguchi University Graduate School of Medicine
|
| Department |
Department of Obstetrics and Gynecology
|
| Street address |
Minami-Kogushi 1-1-1
|
| City |
Ube |
| ZIP/Postal code |
755-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE136616 |
Expression data from non-decidualized and decidualized endometrial stromal cell (ESCs) with or without WT1 knockdown |
|
